Year: 2018 | Month: October | Volume 11 | Issue 5
Single Cell Proliferation to Produce Monoclonal Population of Transfected Caprine Fibroblast Cell
DOI:10.30954/0974-1712.10.2018.2
Abstract:
Transfected cell lines are widely used in biological research. Cell heterogeneity within a population can be vital to its unusual function and fate. Mixed mutants may not be as informative regarding which cell responds to our treatment. Cell to cell differences in RNA transcripts and protein expression further complicate the condition. To understand the real affect on the treated cells, researchers want to have single cell monoclonal population to provide correct information for getting truly transfected cell population. With this fact, we focus on the single cell isolation from transfected cells and its proliferation, which include methods of single cell pickup, analysis of media supplements on growth parameters and optimizing the best condition for single cell proliferation. Dilution method and Single cell pickup with mouth pipette method were compared for their efficacy. Rotational media supplement was found better than the regular medium used for cell growth. To explore the best suitable conditions for cell proliferation and survival, the cell viability percentage, total cell count, cell proliferation rate and population doubling time were calculated at different time interval in different media supplement groups.
© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Print This Article Email This Article to Your Friend
